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Fig. 5 | Retrovirology

Fig. 5

From: Dynamic nanopore long-read sequencing analysis of HIV-1 splicing events during the early steps of infection

Fig. 5

Effect of artificially enhanced splicing at D2 on HIV-1 alternative splicing regulation. Splice trees were drawn on Cytoscape based on ONT quantifications of SS usages and spliced isoform levels in a WT U1 snRNA and b U1D2upEx snRNA conditions. Only expression of transcripts that were found at least 5 times across the replicates and produced by usage of major SS were considered. Usage of SS are represented by nodes, lines symbolize splice junctions between SD and SA sites and the resulting isoforms are represented by triangles. Names of isoforms are indicated only if they could be detected in the condition. Sizes of nodes are function of relative SS usage and sizes of triangles are function of transcript levels as indicated by the scale. As D1 is present in all spliced isoforms, value of D1 was set at 100%. Non-coding exon (NCE) 2 and 3 are indicated

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Retrovirology

ISSN: 1742-4690

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