Fig. 3

Transitional memory and effector memory cells were most frequently infected by C-HIV Envs. a Each data point represents the percentage of infected CD4⁺ T cells with one pseudovirus (averaged from four independent seronegative blood bank donors). The Env donor is indicated as follows; CAP88 (blue circles), CAP177 (red squares), CAP228 (green triangles), CAP255 (purple inverted triangles) and CAP257 (orange diamonds). Black lines represents the median of all pseudoviruses within each time point. Comparisons were made using a Kruskal–Wallis test with Dunn’s post hoc test for multiple comparisons. b Stacked bar graphs represent the contribution of each T cell subset to the pool of infected CD4⁺ T cells. Values represent the median percentage of infected CD4⁺ T-cells (averaged across four HIV-seronegative PBMC donors) that belong to the indicated subset [naïve; dark blue, T stem cell memory (TSCM); red, central memory (CM); yellow, transitional memory (TM); light blue, effector memory (EM); purple and terminally differentiated (TD); green], and are stratified by participant and time point. Error bars represent the interquartile range. c Dot plot representing the proportion of each T cell subset contributing to the total pool of infected cells for all Env-pseudoviruses. Each point represents a single virus averaged across four seronegative donors, lines represent median and error bars represent interquartile range. Comparisons were made using a Kruskal–Wallis test with Dunn’s post hoc test for multiple comparisons and are shown in the table. d Pie charts represent the proportion each CD4⁺ T cell subset contributes to the total pool of infected CD4⁺ T cells for each time point. Each pie slice represents the average of all viruses from T0, T1 and T3 respectively for the indicated T cell subset (naïve; dark blue, TSCM; red, CM; yellow, TM; light blue, EM; purple and TD; green)