Fig. 2

Genotypic characteristics of longitudinal C-HIV Envs during natural infection. a The number of potential N-linked glycosylation sites (PNLGS) within the Acc65I–BamHI fragment of Env stratified by time point (T0, T1, T3) and Env donor indicated as follows; CAP88 (blue circles), CAP177 (red squares), CAP228 (green triangles), CAP255 (purple inverted triangles) and CAP257 (orange diamonds). The b PNLGs, c length in amino acids and d net charge of the V1V2 region within Envs. e PNLGs, f length (amino acids) and g net charge within the V3 region of C-HIV Envs. h PNLGS, i length (amino acids) and j net charge within the V4 region of C-HIV Envs. Black lines represent the median within each time point. For net charge graphs (d, g, and j), the dotted line represents a net charge of 0. Comparisons were made using a Kruskal–Wallis test with Dunn’s post hoc test for multiple comparisons, *p < 0.05, ****p < 0.0001