Fig. 5
From: M-Sec facilitates intercellular transmission of HIV-1 through multiple mechanisms
Effect of M-Sec knockdown on the quantity of HIV-1 in culture supernatants of U87 cells. a U87.CD4.CCR5 (upper) or U87.CD4.CXCR4 cells (lower) were transfected with either control (Cr pool #2) or M-Sec-specific siRNA (#4), and cultured for 2 days. Next, U87.CD4.CCR5 and U87.CD4.CXCR4 cells were infected with JRFL (input: 100 ng/ml p24 Gag) and NL43 (input: 1 ng/ml p24 Gag), respectively, and cultured for 6 days. The concentration of p24 Gag in culture supernatants (day 0, 1, 2, 4, 5, and 6) was determined by ELISA (mean ± SD, n = 3). *p < 0.05. sup, supernatants; dpi, days postinfection. b U87.CD4.CCR5 (upper) or U87.CD4.CXCR4 cells (lower) were transfected with either control (Cr pool #2) or M-Sec-specific siRNA (#4), and cultured for 2 days. Next, U87.CD4.CCR5 cells were infected with the wild-type (WT) or Nef-deficient (ΔNef) JRFL virus (input: 100 ng/ml p24 Gag), and U87.CD4.CXCR4 cells were infected with WT or ΔNef NL43 virus (input: 1 ng/ml p24 Gag). Cells were cultured for 2 days, and analyzed for p24 Gag concentration in supernatants by ELISA (mean ± SD, n = 3). *p < 0.05. n.s. not significant, sup supernatants