Fig. 5
PF74 affects the restriction of MxB to HIV-1. a HIV-1WT and HIV-1N74D were preincubated with PF74 (1 μM) or carrier DMSO and then added to HeLa-Ctrl and HeLa-MxB cells, infectivity was determined 48 h post infection by luciferase assay. Results were a summary of 3 independent experiments. b HeLa cells were transfected with plasmid expressing MxB-HA protein or empty vector. 48 h after transfection, cells were incubated with HIV-1WT in the presence of PF74. Cells were fixed 6 h post infection and stained for HIV-1 capsid protein p24 (green), NUP358 (red) and DAPI (blue) for cell nuclei. Scale bar, 10 μm. c P24 spots, the percentage of the p24 signals detected in the nucleus and the percent p24 colocalizing with NUP358 in HeLa cells was counted in about 40 cells. Data were representative of 3 independent experiments. Statistical significance: NS: not significant, *p < 0.05, **p < 0.01 and ***p < 0.001