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Fig. 4 | Retrovirology

Fig. 4

From: MxB impedes the NUP358-mediated HIV-1 pre-integration complex nuclear import and viral replication cooperatively with CPSF6

Fig. 4

CPSF6 is essential for MxB to inhibit HIV-1 nuclear import in target cells. Primary MDMs (a) and CD4+ T cells (b) isolated from three independent donors were transduced with concentrated lentivirus overexpressing MxB or a control lentivirus (Ctrl) for 72 h, then cells were transfected with CPSF6 siRNA (siCPSF6) either negative control (siNC). 24 h after siRNA transfection, cells were challenged with HIV-1Bal or HIV-1NL4-3. The level of of MxB, CPSF6, Gag and HIV-1 DNA was determined 48 h post infection. The mean ± SD of three technical replicates were shown for each donor

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