Fig. 2
MxB interrupts the NUP358-mediated HIV-1 nuclear import and viral replication. a HeLa-Ctrl and HeLa-MxB cells were transfected with siRNA targeting NUP358 (siNUP358), NUP98 (siNUP98) or negative control (siNC). 48 h after transfection, the expression levels of NUP358 and NUP98 were monitored by western blot. b, c siRNA depleted cells were infected with similar amount of VSV-G pseudotyped HIV-1 luciferase reporter virus bearing either the wild-type (WT) CA or N74D CA mutant. Infectivity was determined 48 h post infection by luciferase assay (b) and WB analysis of p24 (c). Results were a summary of 3 independent experiments. d HeLa cells were transfected with plasmid expressing MxB-HA protein or empty vector. 48 h after transfection, cells were infected with similar amount of HIV-1WT or HIV-1N74D. Cells were fixed 6 h post infection and stained for HIV-1 capsid protein p24 (green), NUP358 (red) and DAPI (blue) for cell nuclei. Scale bar, 10 μm. e P24 spots, the percentage of the p24 signals detected in the nucleus and the percent p24 colocalizing with NUP358 in HeLa cells was counted in about 40 cells. Data were representative of 3 independent experiments. Statistical significance: NS: not significant, *p < 0.05, **p < 0.01 and ***p < 0.001