Fig. 2
pNL4-3 Gag-iNanoLuc enables highly sensitive detection of virus release. a Western blot analysis of HIV-1 Gag in lysates and supernatants from HEK293T cells transfected with 1.0μg pUAS-NanoLuc, 1.0μg pNL4-3, or varying amounts of pNL4-3 Gag-iNanoLuc (1.0μg, 0.5μg, 0.25μg, 0.125μg or 0.0625μg). b NanoLuc activity of lysates and supernatants from HEK293T cells transfected as in a at 48 h post-transfection represented as Log10[RLUs]. c RT activity of supernatants from b. d Relative amounts of p24 in pg/ml in supernatants from (b). e Correlation of supernatant NanoLuc-activity and RT activity from b. f Correlation of supernatant NanoLuc activity and p24 abundance from b. Error bars ± SD, n = 3 independent experiments for panels b, c and d. *, P < 0.05 by one way ANOVA in panels b, c and d compared to 1.0μg pNL4-3 Gag-iNanoLuc in B and WT pNL4-3 RT activity and p24 in c and d, respectively