Fig. 2
From: Functional properties and sequence variation of HTLV-1 p13
Intracellular localization of p13-GFP. a Confocal microscopy analysis of HeLa cells transfected with a p13-GFP-expressing plasmid and labelled with an antibody recognizing the mitochondrial protein HSP60 (Santa Cruz Biotechnology) and an Alexa-546-conjugated secondary antibody (Life Technologies). Nuclei were visualized using Vibrant DyeCycle Ruby (Life Technologies). Nuclei, mitochondria, and p13 are shown in blue, red, and green, respectively. b Quantitative analysis of the percentage of p13-GFP signal detected in nucleus in relation to the total p13-GFP signal measured in the same cell