Fig. 7
From: DDX5 potentiates HIV-1 transcription as a co-factor of Tat
DDX5 glycine doublet is essential for efficient HIV-1 replication. a Schematic representation of DDX5 and the annotated individual point mutations in the glycine doublet motif that is defective for ATPase/helicase activities and protein–protein interactions. b HIV-1 infectivity following DDX5 depletion and rescue with siDDX5A-resistant, glycine doublet motif mutated plasmid, DDX5-G203D. Supernatant from transfected cells was used to infect TZM-bl cells. c Cell lysates from b were harvested and CA-p24 quantified by ELISA. Each graph is a representative of at least two independent experiments done in triplicate. Bars represent mean of triplicate samples ± SEM. Statistical significance **P < 0.01. d Western blot showing the expression of DDX5 after knockdown and rescue with DDX5-G203D