Fig. 6

DDX5 Walker A is dispensable but Walker B (DEAD) helicase activity is required. a Schematic representation of DDX5 and the annotated individual point mutations in the Walker A and Walker B (DEAD) motifs that are defective for ATPase and helicase activity respectively. b HIV-1 infectivity following DDX5 depletion and rescue with siDDX5A-resistant, Walker A motif mutated plasmid, DDX5-K144A. Supernatant from transfected cells was used to infect TZM-bl cells. c Western blot showing the expression of DDX5 after knockdown and rescue with DDX5-K144A. d HIV-1 infectivity following DDX5 depletion and rescue with siDDX5A-resistant, Walker B motif mutated plasmid, DDX5-E249Q. Supernatant from transfected cells was used to infect TZM-bl cells. e Cell lysates from d were harvested and CA-p24 quantified by ELISA. Each graph is a representative of at least two independent experiments done in triplicate. Bars represent mean of triplicate samples ± SEM. Statistical significance *P < 0.05, **P < 0.01, ***P < 0.0001. f Western blot showing the expression of DDX5 after knockdown and rescue with DDX5-E249Q