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Fig. 5 | Retrovirology

Fig. 5

From: DDX5 potentiates HIV-1 transcription as a co-factor of Tat

Fig. 5

DDX5 N terminal RNA binding motifs are indispensable in HIV-1 replication. a Schematic representation of DDX5 and the annotated individual point mutations that are defective for ATPase and RNA binding activities in the Q motif and motifs 1a and 1b. b HIV-1 infectivity following DDX5 depletion and rescue with siDDX5A-resistant, Q motif mutated plasmid DDX5-Q121A. Supernatant from transfected cells was used to infect TZM-bl cells. c HIV-1 infectivity following DDX5 depletion and rescue with siDDX5A-resistant, motif 1a mutated plasmid, DDX5-T176R + E178V + L179A. Supernatant from transfected cells was used to infect TZM-bl cells. d HIV-1 infectivity following DDX5 depletion and rescue with siDDX5A-resistant, motif mutated plasmid, DDX5-T224D. Supernatant from cells transfected with siDDX5A and then a second round of siRNA together with pLAI, with or without increasing concentrations of DDX5-T224D construct, was used to infect TZM-bl cells. e Cell lysates from b were harvested and CA-p24 quantified by ELISA. f Cell lysates from c were harvested and CA-p24 quantified by ELISA. g Cell lysates from d were harvested and CA-p24 quantified by ELISA. Each graph is a representative of at least two independent experiments done in triplicate. Bars represent mean of triplicate samples ± SEM. h Western blot showing the expression of DDX5 after knockdown and rescue with DDX5-Q121A. i Western blot showing the expression of DDX5 after knockdown and rescue with DDX5-T176R + E178V + L179A. j Western blot showing the expression of DDX5 after knockdown and rescue with DDX5-T224D. Statistical significance *P < 0.05, **P < 0.01. See also Additional file 4: Figure S4

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