Fig. 3

DDX5 is specifically required for HIV-1 transcription and interacts with HIV Tat and cellular HEXIM1. a Myc-DDX5 was co-transfected with HIV-1 replication competent proviral construct (pLAI). Cells were harvested after 48 h and subjected to co-immunoprecipitation followed by western blot for HIV Tat. DDX5 co-immunoprecipitates with Tat. Hela whole cell lysates from cells transfected with replication competent HIV-1 proviral plasmid, were run in parallel as positive control for HIV Tat, to the immunoprecipitation treated samples. The blot is a representative of three independent experiments. b Myc-DDX5 was co-transfected with HIV-1 proviral construct (pLAI). Cells were harvested after 48 h and subjected to co-immunoprecipitation followed by western blot for HEXIM1. Representative western blot of three independent experiments showing that DDX5 co-immunoprecipitates with HEXIM1. c TZM-bl cells were transfected with constant amount of Tat expressor plus either siControl or siDDX5A. Cell lysates were harvested after 72 h and luciferase activity measured using the luciferase assay. The graph is a representative of three independent experiments done in triplicate. Bars represent mean of triplicate samples ± SEM. d DDX5 potentiates Tat dependent, HIV-1 LTR-driven, firefly luciferase activity. TZM-bl cells were co-transfected with pcDNA3.0-Tat and increasing concentration of Myc-DDX5. Cells were harvested after 48 h and luciferase activity measured. The graph shown is a representative of three independent experiments done in duplicate ± SEM. e Western blot showing the expression levels of DDX5 in TZM-bl cells. Statistical significance: *P < 0.05. See also Additional file 2: Figure S2 and Additional file 3: Figure S3