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Fig. 2 | Retrovirology

Fig. 2

From: Longitudinal within-host evolution of HIV Nef-mediated CD4, HLA and SERINC5 downregulation activity: a case study

Fig. 2

Function of within-host nef clones. ac Representative flow cytometry plots showing CD4 (blue), HLA (red) and SERINC5 (green) downregulation activities of select participant-derived Nef clones and controls. The numbers in bold within each plot denote the median fluorescence intensity (MFI) of receptor expression in that gate. The number at the bottom of each plot denotes each Nef clone’s function normalized to that of the positive control SF2NEF. d SF2NEF-normalized CD4 downregulation activity of the 50 Nef clones. Each clone was independently assayed a minimum of 3 times; a total of 189 replicates are represented here. e SF2NEF-normalized HLA downregulation activity of the 50 Nef clones (183 total replicates). f SF2NEF-normalized SERINC5 downregulation activity of the 50 Nef clones (157 total replicates). Horizontal bars show mean normalized function for each clone

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