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Table 1 HTLV-1 quantitative immortalization assay

From: HTLV-1 CTCF-binding site is dispensable for in vitro immortalization and persistent infection in vivo

# of producer cellsNumber of wells with immortalized cells (48 wells/assay)
HTLV-1HTLV-1∆CTCFHTLV-1p12Stop
12 (4%)2 (4%)0 (0%)
109 (19%)8 (17%)6 (13%)
10020 (42%)14 (29%)14 (29%)
100031 (65%)27 (56%)28 (58%)
  1. There is no difference between HTLV-1, HTLV-1 p12Stop, and HTLV-1∆CTCF immortalization capacity. Freshly isolated hPBMCs were cocultured with fixed numbers of lethally irradiated viral producer cells in 96-well plates. The number of wells containing proliferating lymphocytes was enumerated.