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Table 1 HTLV-1 quantitative immortalization assay

From: HTLV-1 CTCF-binding site is dispensable for in vitro immortalization and persistent infection in vivo

# of producer cells

Number of wells with immortalized cells (48 wells/assay)

HTLV-1

HTLV-1∆CTCF

HTLV-1p12Stop

1

2 (4%)

2 (4%)

0 (0%)

10

9 (19%)

8 (17%)

6 (13%)

100

20 (42%)

14 (29%)

14 (29%)

1000

31 (65%)

27 (56%)

28 (58%)

  1. There is no difference between HTLV-1, HTLV-1 p12Stop, and HTLV-1∆CTCF immortalization capacity. Freshly isolated hPBMCs were cocultured with fixed numbers of lethally irradiated viral producer cells in 96-well plates. The number of wells containing proliferating lymphocytes was enumerated.