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Fig. 7 | Retrovirology

Fig. 7

From: HTLV-1 CTCF-binding site is dispensable for in vitro immortalization and persistent infection in vivo

Fig. 7

Hbz and Gag/Pol gene expression is maintained in rabbits infected with HTLV-1∆CTCF. a Hbz and b Gag/Pol gene expression was assessed via qPCR. RNA was isolated from rPBMCs, 250 ng of RNA was reverse transcribed, then a 12-cycle cDNA pre-amplification of 2 µL of cDNA was performed followed by a 45-cycle qPCR using 2 µL of pre-amplified cDNA with Hbz- or Gag/Pol-specific primer and probe sets (Table 2). Total copy number was determined using a standard curve generated by duplicate samples of log10 dilutions of Hbz or Gag/Pol standards listed in Table 2. Copy numbers were normalized to 1 × 106 rGAPDH. There was no significant difference in Hbz or Gag/Pol gene expression. Each symbol represents the Hbz or Gag/Pol copy number of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. Bars represent mean Hbz or Gag copy numbers. Rabbits with a copy number of zero are not represented due to log transformation. Hbz gene expression values for two rabbits (week 0 HTLV-1 and week 12 HTLV-1p12Stop) were negative after log transformation and are not graphically represented. A mixed model analysis with a Bonferroni correction was performed in weeks 8 and 12 to determine statistical significance. A p < 0.0083 was considered a statistically significant change

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