Fig. 6
Ablation of HTLV-1 CTCF-binding site significantly decreases HTLV-1-specific antibody response, but not total rabbit IgG. a Antibody response was quantified using a modified Avioq HTLV-1/2 Microelisa System protocol (Avioq, Inc., Research Triangle Park, NC). The supplied horseradish peroxidase (HRP) conjugated goat anti-human immunoglobulin (Ig) was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom). Rabbit plasma was diluted 1:500 to obtain absorbance values within the linear range of the assay. Each symbol represents the absorbance value of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. b Total rabbit IgG was quantified using the Abcam Rabbit IgG ELISA Kit in accordance with the provided protocol (ab187400; Abcam, Cambridge, United Kingdom). Plasma samples were diluted 1:1 × 106. Each symbol represents total IgG of a single inoculated rabbit at 0, 2, or 12 weeks post-infection within each group. Bars represent mean absorbance or IgG values. Mixed model analyses with a Bonferroni correction were performed in weeks 8 and 12 (HTLV-1-specific) or 2 and 12 (total rabbit IgG) to determine statistical significance. A p < 0.0083 was considered a statistically significant change. Significant changes are denoted by a black line