Table 1 Cell lines analyzed in this study from NIH AIDS reagents program and the Greene laboratory
Cell line | Cell lineage | Parental cell line | Virus | Replication competent virus | Viral mutation | Proviral copies reported per cell | Virus expression phenotype | References | |
|---|---|---|---|---|---|---|---|---|---|
U1 | Monocyte | U937 | AC079807.4 non-coding ORF (Chr.X) | HIV-1 | Yes | Mutation in tat | 2 | Minimal constitutive virus expression Inducible with cytokines + PMA | [12] |
ACH2 | Lymphocyte | CEM | NT5C3A | LAV | Yes | Mutation in tar | 1 | Constitutively produces low levels of supernatant RT and p24 Infectious HIV-1 production inducible with PMA or TNFα | |
J-lat 6.3 | Lymphocyte | Jurkat | nd | HIV-R7/E-/GFP | No | Frameshift in env Nef defective | 1 | Minimal constitutive virus expression (secretion of incomplete viral particles) Inducible with PHA or PMA ± ionomycin | [15] |
J-lat 8.4 | Lymphocyte | Jurkat | FUBP1 | HIV-R7/E-/GFP | No | Frameshift in env Nef defective | 1 | Minimal constitutive virus expression (secretion of incomplete viral particles) Inducible with PHA or PMA ± ionomycin | [15] |
J-lat 9.2 | Lymphocyte | Jurkat | PPP5C | HIV-R7/E-/GFP | No | Frameshift in env Nef defective | 1 | Minimal constitutive virus expression (secretion of incomplete viral particles) Inducible with PHA or PMA ± ionomycin | [15] |
J-lat 15.4 | Lymphocyte | Jurkat | UBA2 | HIV-R7/E-/GFP | No | Frameshift in env Nef defective | 1 | Minimal constitutive virus expression (secretion of incomplete viral particles) Inducible with PHA or PMA ± Ionomycin | [15] |
J-lat 5A8 | Lymphocyte | Jurkat | MAT2a | HIV-R7/E-/GFP | No | Frameshift in env Nef defective | 1 | Minimal constitutive virus expression (secretion of incomplete viral particles) Inducible with T cell activation (α-CD3/CD28 antibodies), > 3.3 μg/ml PHA or PMA ± ionomycin | [97] |
8E5 | Lymphocyte | CEM | nd | LAV | No | Frameshift in pol | 1 | Constitutive expression of defective virus particles (no RT), with high p24 expression |