Fig. 2

Binding characterisation of purified bispecific antibodies by ELISA. a HIV-1 Env binding of bNAbs and bibNAbs were assessed against different immobilized HIV-1 Env conformations (gp140_FVCGN4, gp120_FVCMonomer, gp140_FVCSOSIP) and b the N-terminal two domain fragment of human CD4 (2dCD4) to assess their binding capabilities, specificity and bispecificity or lack thereof. The bNAbs and bibNAbs were tested at a concentration of 1 µg/ml except for VRC01, which was tested at 0.5 µg/ml. Secondary antibody concentration of 1 in 1000 was used for CAP256, iMab-CAP256, 10E08 and 10E08-iMab. For iMab and VRC01, a secondary antibody concentration of 1 in 2000 was used