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Fig. 1 | Retrovirology

Fig. 1

From: Engineering and characterising a novel, highly potent bispecific antibody iMab-CAP256 that targets HIV-1

Fig. 1

Bispecific antibody design, purification and SDS-PAGE analysis. a Left panel: Schematic of the iMab-CAP256 or 10E08-iMab bibNAb, with ‘knob’ mutations introduced into the CH3 region of CAP256 or 10E08 and complementing ‘hole’ mutations introduced into the CH3 region of iMab. Additionally, the CH1 region and the CL region of iMab were interchanged to create a bibNAb with a CrossmabCH1-CL configuration. a Right panel: Schematic of iMab engineering using knob-in-a-hole and CrossMabCH1-CL to serve as a suitable assembly control. b Size exclusion chromatograms of Protein-A purified antibodies. Shaded rectangles represent collected and pooled fractions corresponding to the monomeric antibody conformation. c SDS-PAGE analysis of antibodies under reducing and non-reducing conditions post size exclusion chromatography. Purified human IgG was included as a positive control. Molecular weight (MW) is indicated in kDa

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