Fig. 6
From: Effect of induced dNTP pool imbalance on HIV-1 reverse transcription in macrophages
Effect of dNTP pool imbalances on transduction of SIVmac239 GFP vector in macrophages. Human primary monocyte-derived macrophages prepared from four healthy donors (a) dNTP levels were measured after macrophages were first treated with VLPs with (+) and without (−) Vpx for 12 h and then with 1 mM dA or dG for 4 h. The dNTP levels in these cells were determined by the RT-based dNTP assay. “+” indicates the treated dNTPs, and “x” indicates untreated dNTPs. NT No dN treatment. b Macrophages were treated with various biased dN as described in Fig. 1 (dG, dA or dT), 2 (dG + dA + dT), 3 (dC), or Additional file 1: Figure S1 “All” (dG, dA, dC or dT). The treated cells were transduced with SIVmac239-GFP vector, and transduction efficiency was measured via percent of GFP+ cells. The transduction efficiency for each treatment was normalized to the transduction efficiency with no dN treatment. The data are the mean of three independent experiments and error bars represent the standard deviation from the mean. *p-value < 0.05