Fig. 4

Biochemical simulations of HIV-1 RT mediated DNA synthesis under the induced dNTP pool imbalance. a 5′ ³²P-labeled 18-mer DNA primer (P) annealed to a 26-mer template DNA (T) was used in the HIV-1 RT mediated primer extension reaction. The 5′ ³²P-labeled primer (2 nM) annealed to the template was extended by purified HIV-1 RT protein for 5 min at 37 °C with the dNTP concentrations determined in macrophages with b single dNTP elevation (dG, dA, dT or dC treatment, from Figs. 1a and 3a) and single dNTP depletion (dG + dA + dT, “GAT” treatment from Fig. 2a). NT: dNTP concentrations from untreated macrophages (Fig. 1a). The concentrations of individual dNTPs in each reaction are shown in Additional file 1: Table S1. F fully extended 26-bp product, P 18-mer unextended primer