TY - JOUR AU - Shepard, Caitlin AU - Xu, Joella AU - Holler, Jessica AU - Kim, Dong-Hyun AU - Mansky, Louis M. AU - Schinazi, Raymond F. AU - Kim, Baek PY - 2019 DA - 2019/10/26 TI - Effect of induced dNTP pool imbalance on HIV-1 reverse transcription in macrophages JO - Retrovirology SP - 29 VL - 16 IS - 1 AB - Terminally differentiated/nondividing macrophages, a key target cell type of HIV-1, harbor extremely low dNTP concentrations established by a host dNTP triphosphohydrolase, SAM domain and HD domain containing protein 1 (SAMHD1). We tested whether the induction of dNTP pool imbalance can affect HIV-1 replication in macrophages. For this test, we induced a large dNTP pool imbalance by treating human primary monocyte derived macrophages with either one or three of the four deoxynucleosides (dNs), which are phosphorylated to dNTPs in cells, to establish two different dNTP imbalance conditions in macrophages. SN - 1742-4690 UR - https://doi.org/10.1186/s12977-019-0491-0 DO - 10.1186/s12977-019-0491-0 ID - Shepard2019 ER -