Fig. 5

FUS non-specifically associates with TAR RNA. Jurkat (J)-LTR-Luc-FUS and J-LTR-Tat-FUS cells that harbor the integrated HIV-LTR-Luc reporter and also over-express Flag-FUS were subjected to RNA immunoprecipitation (RIP) qPCR. Cell lysate was immune-precipitated with either anti-Flag-IgG (black bars), or control non-specific IgG (gray bars). RNA was then extracted from IP or input (10%) samples with Trizol, followed by reverse transcription and amplification with primers that target the indicated RNA. qPCR reactions on samples were performed in triplicates and presented as percentage from input ChIP material. The error bars represent mean ± SD of the triplicate independent qPCR reactions. Asterisks indicate levels of statistical significance calculated by two-tailed student T test (p < 0.01)