Fig. 5

CCR5 disruption via LentiCRISPR/SaCas9 renders CD4⁺ T cells survival from HIV-1 infection in vivo. a Scheme of humanized mice experiment. Experiment schedule and process were showed at each time point. 16 mice were divided into two groups randomly and each group received mock-modified or SaCas9/sgRNA-#8-CCR5-modified human CD4⁺ T cells. b Assess engraftment of all the mice post injection by flow cytometry to calculate CD4⁺ T cell counts. c Analysis of CD4⁺ T cell counts after infection with HIV-1. CD4⁺ T cells counts were calculated at 14, 28, 45 days post-infection by flow cytometry. d Measure the proportion of human CD4⁺ T cells in spleens of HIV-1 infected mice after euthanasia. e The relative copy numbers of gag gene in CD4⁺ T cells of spleens in d. f CCR5 alleles mutation in CD4⁺ T cells from humanized mice. The human CD4⁺ T lymphocytes from spleens were conducted for genomic DNA extraction and T7E1 assay after 45 days infection. Mutation were showed in CCR5-modified CD4⁺ T cells in mice. Data were analyzed by unpaired t-test. Error bars showed the mean ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001)