Fig. 5

LEDGIN treatment during virus production hampers reactivation of residual integrants. Eight days post infection with OGH virus produced in presence of LEDGINs, cells were reactivated in duplicate with 10 ng/µl TNFα for 24 h followed by flow cytometry analysis. a, c The quiescent fraction (C/(A + B + C) × 100, Fig. 2b) is depicted for unactivated cells in gray and activated cells in green for three different virus dilutions in Jurkat (a) and SupT1 cells (c). b, d The decrease in quiescent fraction between non-activated and TNFα-treated cells is shown for three different virus dilutions in Jurkat (b) and SupT1 cells (d). Data represent averages of duplicates with standard deviation from a representative experiment (total five experiments in Jurkat cells and ten in SupT1 cells). Statistical significance was determined for all dilutions at each concentration of LEDGINs compared to the DMSO control via a one-way ANOVA with Dunnet’s multiple comparison test (**p < 0.01, ***p < 0.001). TNFα Tumor Necrosis Factor alpha, DMSO dimethyl sulfoxide