Fig. 2

The double reporter virus allows characterization of quiescent and productive infection. a Schematic representation of the two-colored OGH reporter virus encoding eGFP in the Nef position driven by the viral LTR promoter and carrying a constitutive transcriptional unit (EF1α-mKO2) inserted downstream [61, 62]. b Representative dot plot after flow cytometry analysis of infected SupT1 cells showing the characterization of productive and latent populations based on fluorescence. All infected cells express mKO2 (mKO2⁺). If cells are productively infected, the viral LTR promoter will drive eGFP expression resulting in double positive cells (quadrant B; eGFP⁺, mKO2⁺). Cells only showing mKO2 expression are considered to be quiescently infected (quadrant C). c Flow cytometry analysis of infected SupT1 cells (virus dilution 1/4000 and 1/8000) treated with a dilution series of LEDGIN CX014442 during infection. eGFP enhanced Green Fluorescent Protein, mKO2 mutant Kusabira Orange 2