Fig. 5
From: Functional analysis of the secondary HIV-1 capsid binding site in the host protein cyclophilin A
Functional analysis of CypA mutants in Hela-P4 cells. The PPIA gene was disrupted in Hela-P4 cells with CRISPR technology. The cloned cell line was transduced with a lentiviral vector encoding the indicated CypA mutant cDNAs. a Analysis of the extent of HIV-1 infection of the indicated cell lines by the A92E CA mutant in the presence and absence of CsA. A higher ratio indicates less enhancement of infection by CsA. Shown are the results of two independent determinations. b Quantitative analysis of CypA expression by immunoblotting relative to wild type HeLa-P4 cells. All samples were separated on the same gel and CypA and GAPDH were stained on the same blot membrane. CypA signals were normalized by the corresponding GAPDH signals