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Fig. 11 | Retrovirology

Fig. 11

From: Analysis of herpes simplex type 1 gB, gD, and gH/gL on production of infectious HIV-1: HSV-1 gD restricts HIV-1 by exclusion of HIV-1 Env from maturing viral particles

Fig. 11

The HSV-1 gD co-localizes with Gag-EGFP. 293 cells were transfected with either the vector expressing gD, Gag-EGFP, or co-transfected with gD and Gag-EGFP. At 24 h, cells on cover slips were washed, fixed, permeabilized and blocked as described in the Materials and Methods section. The cover slips were reacted with a mouse monoclonal antibody against gD and an appropriate secondary antibody and counterstained with DAPI (1 μg/ml) for 5 min. The cover slips were mounted and examined using a Leica TCS SPE confocal microscope using a 63X objective with a 2X digital zoom using the Leica Application Suite X (LAS X, LASX) software package. A 405 nm filter used to visualize the DAPI, 488 nm filter was used to visualize the Gag-EGFP, and a 594 nm filter to visualize gD staining. a–d Cells transfected with vector expressing gD. e–h Cells transfected with vector expressing Gag-EGFP. i–l Cells co-transfected with vectors expressing gD and Gag-GFP. a, e, i Visualization of DAPI staining. b, f, j Visualization of gD staining. c, g, k Visualization of Gag-EGFP. d, h, l Merge of the three panels to the left

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