Fig. 5

Hypericin-PDT suppressed the expression of viral genes and inhibited HTLV-1 LTR activity. a Expression of HBZ transcripts was detected by qPCR in HPB-ATL-T and TL-Om1 cells after treating with hypericin-PDT, b HPB-ATL-T cells were treated with hyerpicin-mediated PDT for 24 h. The expression of Tax and GAPDH were analyzed by western blot, c hypericin-PDT impaired the HTLV-1 5′LTR and 3′LTR promoter activity. Cells were transfected with the Tax and HTLV-1 LTR reporter plasmids. Luciferase activity was measured 48 h after hypericin-PDT treatment, d hypericin-PDT inhibited Tax DNA binding capability. 293FT cells were transfected with FLAG-Tax together with WT-Luc or 3′LTR-Luc. After hypericin-PDT treatment, 293FT cells were chromatin immunoprecipitated by anti-FLAG antibody. The precipitated DNAs and 1% of the input cell lysates were amplified using the WT-Luc or 3′LTR-Luc specific primers by real-time PCR. Statistically significant differences are labeled *P < 0.05, **P < 0.01, compared with control group using Student’s t-test