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Fig. 1 | Retrovirology

Fig. 1

From: Hypericin-photodynamic therapy inhibits the growth of adult T-cell leukemia cells through induction of apoptosis and suppression of viral transcription

Fig. 1

Hypericin-PDT induced growth arrest in ATL cells. a The effects of hypericin-PDT treatment on the growth of HTLV-1-positive cell lines (HPB-ATL-T, MT-2, C8166, and TL-Om1) and HTLV-1-negative T-cell line (CEM-T4). Cells were treated with increasing amounts of hypericin with or without light irradiation for 24 h. The proliferation of each cell was examined by methyl thiazolyl tetrazolium assay. HY indicates hypericin, and HY + L indicates hypericin with light irradiation, b influence of hypericin on colony forming efficiency of HPB-ATL-T cells. (Left panel) I: control group; II: 50 ng/mL hypericin-PDT group; III: 100 ng/mL hypericin-PDT group. (Right panel) Quantitative representation of colony forming efficiency on HPB-ATL-T cells, c resting and activated CD4+ T lymphocytes are resistant to hypericin-PDT. CD4+ T cells were isolated from PBMCs of healthy donor. Activated CD4+ T cells were supplemented with 10 ug/mL PHA. Cells were treated with hypericin with or without light irradiation up to 24 h. Cell growth was assayed in triplicate wells by MTT assay, d HTLV-1 infected Jurkat cells are sensitive to hypericin-PDT treatment. Jurkat were transfected with pX1MT-M by electroporation using Neon. Cells were treated with the indicated concentration of hypericin with or without light irradiation for 24 h. Cell growth was assayed by MTT assay. All statistical analyses are shown as *P < 0.05; **P < 0.01. Representative results of 3 independent experiments are shown

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