Fig. 4

UPF2 and SMG6 knockdown enhance HIV-1 vRNA expression in primary HIV-1-infected MDMs. Human monocytes were differentiated into MDMs and then transfected with control siRNA (siNS) or siRNAs directed against UPF1, UPF2 or SMG6. After 24 h, cells were infected with NL4-3-Bal-IRES-HSA virus (MOI: 1.0) and kept in culture for 6 days. a Fold change in the levels of vRNA as measured by RT-qPCR and normalized to the siNS HIV-1 + condition. Error bars represent the standard deviation from two independent experiments with cells from three different donors each (One-way ANOVA; ns: not significant, *p ≤ 0.05 and p ****p ≤ 0.0001). b Cells were transfected with siNS, siUPF1, siUPF1 or siUPF1 and siUPF2 combined, infected and after 6 days were collected, incubated with anti-HSA antibody and analysed by flow cytometry. Fold change in the HSA expression was normalized to the siNS condition. Error bars represent the standard deviation from three independent experiments with cells from one donor. Asterisks represent statistically significant difference between groups (One-way ANOVA; *p ≤ 0.05)