Fig. 1

UPF1, UPF2, and SMG6 expression are reduced in HIV-1-infected MDMs. Human monocytes were differentiated into MDMs and infected with NL4.3-Bal-IRES-HSA virus (MOI: 1.0). Cells were collected at 6 days post-infection, incubated with anti-HSA antibody and sorted through magnetic separation as described previously [44]. a Schematic of the sorting strategy to separate HSA-negative from HSA-positive cells. b Cell lysates were run on SDS-PAGE gels and UPF1, UPF2, SMG6, pr55^Gag and actin protein levels were detected by Western Blotting. c Fold changes in expression levels of each protein between bystander and HIV-1-infected cells. Error bars represent the standard deviation from three independent experiments with cells from three different donors each. Asterisks represent statistically significant difference between bystander and infected cells (One-way ANOVA; **p ≤ 0.01 and p ***p ≤ 0.001)