Fig. 7
From: Sequential trafficking of Env and Gag to HIV-1 T cell virological synapses revealed by live imaging
Live images of co-transfer of Gag and Env containing puncta from a donor cell to a target cell. Donor cells were Jurkat cells co-transfected with Gag-iCherry and Env-isfGFP-∆V1V2. Target cells, primary peripheral blood CD4 T cells, were mixed with donor cells at 24 h post transfection. Confocal z stacks were acquired at 3-min intervals from 0.5 h post co-culture for 3 h. Bar = 8 μm