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Fig. 6 | Retrovirology

Fig. 6

From: Sequential trafficking of Env and Gag to HIV-1 T cell virological synapses revealed by live imaging

Fig. 6

Redistribution of Gag to the virological synapse can be observed after Env accumulation. Time-lapse confocal fluorescence images show the process of VS formation. The donor cell expresses both Gag-iCherry and Env-isfGFP-∆V1V2. Confocal z-stacks were acquired at 3-min intervals from 0.5 h after donor cell and target cell mixing. From 0 to 6 min, donor-target cell adhesion is visualized and Env accumulation was observed (arrow) at the site of cell–cell contact. At t = 0 min, Gag was evenly distributed across the plasma membrane. At t = 3 min, Gag begin to re-distribution and at t = 6 min, increased Gag localization at contact site is evident. At t = 36 min, prominent Gag accumulation at the VS was observed but Env was no longer observed. Independent events observed N = 5. RLT: reference lookup table; Bar = 5 μm

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