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Fig. 4 | Retrovirology

Fig. 4

From: Clonal anergy of CD117+chB6+ B cell progenitors induced by avian leukosis virus subgroup J is associated with immunological tolerance

Fig. 4

B cell function and responses to antigens and pathogens were impaired in ALV-J congenitally infected chickens. a chickens infected by ALV-J at ED 6 showed extremely weakened responses in IgM and IgG classes against both SRBC and MDV, but chickens infected at D 1 had normal values for IgM class Abs. **p < 0.01, ***p < 0.001, two-way ANOVA was performed. b IHC staining for chB6 antigen in tissue sections of spleen using mouse anti-chB6 Ab. The numbers of splenic GCs (red circles) and B cells in infected chickens after LPS challenge did not change compared with pre-LPS challenge. However, there were significant differences between the uninfected chickens (n = 6) and infected chickens (n = 6). Sections were stained by BCIP/NBT (black purple) and counterstained by haematoxylin (magnification: ×100). c Quantitation of b; **p < 0.01, two-way ANOVA was performed

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