Fig. 6
From: RNA-induced epigenetic silencing inhibits HIV-1 reactivation from latency
TGS-inducing shRNAs affect the epigenetic profile of the HIV-1 LTR during TNF reactivation. Parental and transduced J-Lat 9.2 cell lines were stimulated for 48 h with 5 ng/mL of TNF, and ChIP assays performed on sorted live GFP- and GFP + populations. The statistical significance of immunoprecipitation levels of a H3K27me3, b H3K9me2, c H3K9me3, and d H3K9Ac, during HIV-1 latency (left panels) and HIV-1 reactivation (middle panels), was analyzed by performing “in-between” cell lines multiple comparisons against Parental (dark-grey bar). Multiple comparisons “in-within” cell lines were also performed to examine significant changes from latency to reactivation within each cell line (right panels). Data shows Mean ± SD (n = 4) from two independent ChIP assays and are presented as the % Input normalised to Parental (* = p values from 0.05 to 0.01, ** = p values from 0.009 to 0.001, *** = p values from 0.0009 to 0.0001 and **** = p values < 0.0001)