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Fig. 3 | Retrovirology

Fig. 3

From: RNA-induced epigenetic silencing inhibits HIV-1 reactivation from latency

Fig. 3

GFP+ cells expressing protective shRNAs expressed reduced levels of GFP. Parental and transduced J-Lat 9.2 cells were treated with increasing concentrations of a TNF, b SAHA, c SAHA and TNF, d Bryostatin and TNF, e Chaetocin, f DZNep. All treatments were assessed at 48 h, except from Chaetocin and DZNep, which were assessed at 72 h. Expression of GFP was measured through flow cytometry as the mean fluorescence intensity (MFI) from the population of GFP + cells gated from the live (Parental) or live/mCherry + cells (transduced cell lines), after collecting 10,000 live events. The clinically relevant concentrations of TNF and SAHA are indicated in the corresponding plots. Comparisons shown were performed against the Parental cell line using the Kruskal–Wallis Multiple comparison test, correcting for Multiple comparison with Dunn’s test (*p ≤ 0.05)(Mean ± SD). Data shown is from at least one independent representative experiment performed in triplicates

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