Skip to main content
Fig. 1 | Retrovirology

Fig. 1

From: RNA-induced epigenetic silencing inhibits HIV-1 reactivation from latency

Fig. 1

Protective shRNAs inhibit HIV-1SF162 replication in Jurkat E6 cells. a Diagram illustrating the conduct of experiments. For simplicity, Parental and E6 untransduced cell lines are not indicated, however these cells underwent the same experimental procedures with the exception of the gating strategy for flow cytometry, as these do not express mCherry protein. b Upper left panel: Parental and transduced Jurkat E6 cells were infected with 375 μU/mL of HIV-1SF162 for 10 days and RT-assays performed in duplicates at the indicated time points post-infection. Lower left panel: Cells were harvested at D10 and cell-associated HIV-1 gag-mRNA was quantitated in duplicates via qRT-PCR and were normalised against GAPDH mRNA. The dotted line at 10° = 1, indicates the level at which transcription of GAPDH and HIV-1 gag-mRNA is equivalent. Upper right panel: Schematic of chromatin structure following siRNA treatment. Lower right: Levels of integrated HIV-1 were quantitated at D10 using a HIV-1 nested real-time Alu PCR. Second-round PCRs were performed in triplicates and are presented normalised to β-actin. Data are presented as the number of HIV-1 integrated DNA copies per 500 ng of DNA, with 500 ng equivalent to the DNA amount of 80,000 cells. Data are from one time-course performed with duplicate biological replicates and are shown as Mean ± SD

Back to article page