Fig. 2

Identification of the domain responsible for Tat and RelB interaction. a Schematic representation of Tat and deletion mutants in the context of EGFP fusion proteins. The ability of each mutant to bind to RelB (see experimental results in Fig. 2b) is indicated on the right. The red box indicates the core domain that interacts with RelB. b pFlag-RelB (3 μg) was transiently transfected into HEK 293T cells (4 × 10⁶) together with the indicated wild type or truncated Tat DNA (3 μg). Tat proteins were immunoprecipitated (IP) (anti-EGFP) and immunoblotted with the indicated antibodies. c Schematic representation of RelB protein and its deletion mutants carrying a Flag tag. The ability of each mutant to bind to Tat (see experimental results in Fig. 2d) is shown on the right side. The red box partly covers the RHD domain of RelB, which binds Tat. d HEK 293T cells (4 × 10⁶) were transfected with pEGFP-Tat DNA (3 μg) and the indicated Flag-tagged deletion mutants of RelB (3 μg). RelB was immunoprecipitated (IP) (anti-Flag) and immunoblotted with the indicated antibodies