Stabilizing HIV-1 envelope glycoprotein trimers to induce neutralizing antibodies

Retrovirology

Table 1 Amino acid substitutions that stabilize soluble native-like trimers

	Hydrophobic	Aromatic	Proline/glycine	Disulfide bonds	Charged	Other
C1				E49C-L555C^a	E47D^b	E106T^b
				H72C-H564C^a	K49E^b
				A73C-A561C^a	E64K^c
					V65K^b
					H66R^c
					T106E^d
V1	L154M^e	Y177W^e			S164E^b
V2	I165L^b				Q171K^f
	I172V^f				I192R^f
C2	A204I^g	I203F^h		I201C-A433C^m,n
	M271I^d	F223W^d
	F288L^d
	T290A^d
V3	N300M^e	N302Y/F^j			H308R^b
	N302M^e	A316W^c
	R304V^d	A319Y^d
	S306Lⁱ
	R308Lⁱ
	T320L^e
C3						N363Q^d
C4	Y420M^e			I201C-A433C^m,n	E429R^b	R432Q^b
C5				A501C-T605C^k	A500R^b
FP^p					F519R^j	F516S^d
					L520R^j
HR1^q	D589V^g	T538F^h	L556P^g	E49C-L555C^a	L568D^d	I535N/M^c,g,o
		I548F^h	I559G/P^l	H72C-H564C^a	K588E^g	L543N^b,c
		I573F^g	A561P^d	A73C-A561C^a	G588R^b	N553S^b
			L568G^j			V570H^d
			T569G/P^j,l			R585H^d
						K588Q^g
DSL^r				A501C-T605C^k
	K655I^g	E647F^g	M629P^h
HR2^s	K658V^g	N651F^g	S636G^j
	E662A^b

ISSN: 1742-4690