Fig. 2
From: Development of broadly neutralizing antibodies in HIV-1 infected elite neutralizers
HIV and bNAb co-evolution studies. Longitudinal PBMCs samples are used for bNAb isolation (by functional screening of single B-cell micro-culture and/or antigen-specific cell sorting) and NGS sequencing of the memory B-cell repertoire. In parallel, corresponding longitudinal plasma samples are used to sequence and clone viral env variants. NGS data are used to re-construct the Ab and Env phylogenies over the course of infection. Cloned bNAbs and Env variants are functionally and structurally evaluated, both individually and in complex, to retrace the evolution of the virus-antibody interaction from elicitation to acquisition of neutralization breadth and inform vaccine design