Fig. 4

CrossMab format for bispecific antibody production. Knob-in hole mutations in the CH3 domains favor heterodimer heavy chain formation. CH1-CL crossover in one arm of the CrossMAb favors proper light chain association with its cognate heavy chain. In combination, a intact molecule production and secretion is favored and b byproduct production and secretion is disfavored. Dashed blue circles indicate target domains that, when detected simultaneously, ensure a greater percentage of intact molecule