Fig. 2
Improved solubility and antiviral activity of 10E8.4/iMab. a Apparent solubility and b turbidity of 10E8.4/iMab and its predecessor variant 10E8.2/iMab. c Percent of a 118 Tier-2 HIV-1 Env pseudovirus panel neutralized (based on IC50 values) by 10E8.4/iMab and its predecessor variants 10E8/iMab and 10E8.2/iMab. Parental mAbs iMab and 10E8 are included for reference. d Decrease in viral load by 10E8.4/iMab and its predecessor variant, 10E8.2/iMab, in HIV-1-infected humanized mice. Shaded area indicates the period of weekly antibody administration. Error bars = SD. * = N297A mutant variant of each bispecific antibody. As reported previously [84], this mutation in the Fc region of each bispecific antibody is required for evaluation of non-FcR binding human antibodies in the murine model