Fig. 4

Myricetin inhibits early oligomerization, fibril extension and degradation of PAP248–286. a The effects of myricetin on the oligomerization of PAP248–286, as assessed using 16% Tricine-SDS-PAGE and Coomassie blue staining. PAP248–286 monomer at 500 μg/ml with or without cross-linking served as the control. b The effects of an irrelevant flavonoid (phlorizin) on the oligomerization of PAP248–286. c The effects of myricetin on the oligomerization of GST. d The effects of myricetin on fibril extension by PAP248–286. Mature SEVI fibril seeds (1% wt/wt) were mixed with PAP248–286 peptide (2 mg/ml) in the presence or absence of myricetin (200 μg/ml), and the mixture was agitated at 1400 rpm at 37 °C. Aliquots were collected at various time points and assessed by ThT fluorescence. e Dose–response curve for myricetin inhibition of PAP248–286 (2 mg/ml) fibrillization after 48 h of agitation by ThT assay. f Dose–response curve for myricetin inhibition of PAP248–286 (2 mg/ml) fibrillization seeded by preformed SEVI fibrils (10% wt/wt) after 48 h of agitation by ThT assay. IC₅₀ values were calculated. The values shown represent the mean ± SD (n = 3)