Fig. 1

Myricetin inhibits the assembly of PAP248–286. PAP248–286 (3 mg/ml) was incubated with myricetin (75, 37.5, 18.75, 9.375 and 0 μg/ml), and the mixture was agitated at 1400 rpm at 37 °C. Samples were collected at various time points (0, 6, 12, 18, 24 and 48 h) and monitored by ThT (a) or Congo red staining (b). Average values (± SD) were calculated from triplicate measurements; the data shown represent one representative trial of three independent experiments. c Amyloid fibril samples (500 μg/ml) in the presence or absence of myricetin (8 and 16 μg/ml) at various time points (0, 24 and 48 h), as visualized by TEM. The scale bar is 200 nm. d, e The secondary structure of PAP248–286 or SEVI fibrils (300 μg/ml) in the presence or absence of myricetin (5 μg/ml) at various time points (0 and 24 h) was measured using CD spectroscopy