Fig. 5

Restoration of conformation/antigenicity in 4-2.J41₇₅₃ Env. a Binding of CT-deleted mutant Envs expressed on 293T cells to bNAbs, PGT151 (trimer-selective, cleavage-specific) and VRCO1 (cleavage non-specific) at 20 µg/ml antibody concentration by FACS based cell surface staining assay. The error bars indicate the standard mean obtained from two independent experiments run in duplicates, P < 0.0001. b Ratio of binding to PGT151 versus VRC01 antibodies by different CT deleted mutants in cell-surface binding assay. c Binding of 4-2.J41ΔCT₇₅₃ mutant (upper panel) to CD4bs-directed bNAb, VRC01, glycan directed, V1/V2 conformation-dependent bNAbs, PG9 and PG16; gp41-directed bNAbs, 10E8 and 4E10 over a range of concentrations. Binding of 4-2.J41ΔCT₇₅₃ mutant to non-NAbs, b6, 17b, 2.2B and 447-52D (lower panel). 4-2.J41_WT was used as positive control. d ELISA to determine the sCD4-induced gp120 shedding from 4-2.J41ΔCT₇₅₃ Env transfected 293T cells incubated with and without sCD4 (40 µg/ml). 4-2.J41_WT Env was used as positive control