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Fig. 2 | Retrovirology

Fig. 2

From: Cell surface ectodomain integrity of a subset of functional HIV-1 envelopes is dependent on a conserved hydrophilic domain containing region in their C-terminal tail

Fig. 2

CT-deleted 4-2.J41 Env is cleaved. a Western blot analysis of cell lysates (left panel) and neutravidin-agarose precipitates (right panel) of cell surface biotinylated mock transfected, 4-2.J41WT and 4-2.J41ΔCT711 Env transfected 293T cell lysates are shown. b Spontaneous and sCD4-induced gp120 shedding of 4-2.J41WT and 4-2.J41ΔCT711 Env transfected 293T cells incubated separately with 20 ug/ml of sCD4 determined by ELISA. c FACS-based binding of 4-2.J41WT and 4-2.J41ΔCT711 on cell surface to trimer-selective, cleavage-specific PGT151 bNAb. The Env, 4-2.J41SEKS, a cleavage defective, non-native form of 4-2.J41 Env was used as a negative control. The error bars indicate the standard mean obtained from three independent experiments performed in duplicates, P < 0.0001

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