Quantitation of the latent HIV-1 reservoir from the sequence diversity in viral outgrowth assays

Retrovirology

Table 1 Comparison of estimates and 95% confidence/credibility intervals for IUPMStats and IUPMBayes

Positives	n	IUPMStats			IUPMBayes
Positives	n	Estimate	Lower 95%	Upper 95%	Median	Lower 95%	Upper 95%
0	3	0.17*	0	0.75	0.34	0.05	1.10
1	16	0.29	0.04	2.06	0.61	0.15	1.68
2	28	0.69	0.17	2.85	1.04	0.36	2.41
3	37	1.39	0.41	4.72	1.36	0.52	2.87
4	16	∞	Undefined		1.85	0.80	3.63

We summarized the results of each method on 100 simulations of 4 replicate wells with \(10^6\) cells each, where the true IUPM was set to 1.0 with five sequence variants of equal frequency (1:1:1:1:1). We obtained results for the IUPMStats method directly from the online calculator. Entries for IUPMBayes were averaged across replicate simulations for each number of positives. *IUPMStats uses a median posterior estimate instead of the maximum likelihood estimate (0) when none of the wells are positive

ISSN: 1742-4690