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Fig. 7 | Retrovirology

Fig. 7

From: The RNA surveillance proteins UPF1, UPF2 and SMG6 affect HIV-1 reactivation at a post-transcriptional level

Fig. 7

UPF1 knockdown leads to reduced vRNA levels and Gag expression in primary HIV-1 infected CD4+ T cells. Primary CD4+ T cells were either transduced with shNS or shUPF1-containing lentiviral particles and either left uninfected or infected with HIV-1. a Cell lysates were run on SDS-PAGE gels and UPF1 and pr55Gag protein levels were detected by Western Blotting. b The % of vRNA expressing cells were quantified and normalised to shNS HIV-1-infected condition. Error bars represent the standard deviation from six independent experiments (three donors in duplicate) with at least 5,000,000 cells counted per experiment. Asterisks represent statistically significant difference between groups (Two-way ANOVA; p < 0.001). c Example dot plot depicting vRNA expression in HIV-1 infected shNS and shUPF1 conditions using FISH-Flow technique and, d The % of Gag expressing cells were quantified and normalised to shNS HIV-1-infected condition. Error bars represent the standard deviation from nine independent experiments (three donors in duplicate) with at least 5,000,000 cells counted per experiment. Asterisks represent statistically significant difference between groups (Two-way ANOVA; p < 0.01)

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